Acute pancreatitis (AP) remains a major clinical challenge without targeted pharmacologic therapy. This study established a cerulein-induced murine model to characterize cytokine responses and evaluate IL-6 inhibition as a therapeutic strategy. Mice received hourly cerulein injections followed by isotype or anti–IL-6 antibody treatment. Histology confirmed edema and inflammatory infiltration in pancreata and lungs of cerulein-treated animals. Luminex cytokine profiling showed markedly elevated IL-6, IL-10, KC, MCP-1, MIP-1β, and IL-12 in untreated AP mice. Anti–IL-6 therapy significantly reduced circulating IL-6 and multiple associated mediators, suggesting a downstream anti-inflammatory effect. Serum IL-6 levels differed significantly between groups (p = 0.00044). Future plans include expanding cytokine profiling, quantifying histologic injury over multiple timepoints, and validating IL-6 blockade as a potential therapeutic avenue. This model provides a platform for evaluating cytokine modulation in AP.